The effect of nuclease on transformation efficiency in Serratia marcescens

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An Extracellular Nuclease from Serratia marcescens

The specificity of an extracellular nuclease from Serraiia marcescens was examined with both RNA and DNA as substrates. The enzyme was found to act in a predominantly endonucleolytic manner with less than 2% of the degradation products being mononucleotides. Both DNA and RNA were degraded to di-, tri-, and tetranucleotides terminating in 5’-phosphoryl ends. The enzyme had the unusual property o...

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Effect of Ph on the Formation of Exocellular Nuclease in Aging Broth Cultures of Serratia Marcescens.

Eaves, George N. (Wayne State University College of Medicine, Detroit, Mich.) and Charles D. Jeffries. Effect of pH on the formation of exocellular nuclease in aging broth cultures of Serratia marcescens. J. Bacteriol. 85:1194-1196. 1963.-The formation of exocellular nuclease by Serratia marcescens occurred in the stationary phase of growth and was not associated with cell multiplication. An in...

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ژورنال

عنوان ژورنال: FEMS Microbiology Letters

سال: 1990

ISSN: 0378-1097

DOI: 10.1016/0378-1097(90)90076-3